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Registros recuperados: 62
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Effets de la contamination chimique : des organismes en danger ? ArchiMer
Poisson, Emeline; Fisson, Cédric; Amiard-triquet, Claude; Burgeot, Thierry; Couteau, Jérôme; Dur, Gael; Durand, Fabrice; Forget-leray, Joelle; Letendre, Julie; Souissi, Sami; Xuereb, Benoit.
Le présent fascicule a pour objectif d’apporter les éléments de réponse disponibles pour l’estuaire de la Seine sur ce questionnement. Dans la première partie écotoxicologie. Les effets de la contamination chimique à différents niveaux d’organisation biologique, sur les organismes présents dans l’estuaire de la Seine, seront présentés dans la deuxième partie puis synthétisés dans notion d’analyse du risque environnemental.
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00319/43019/42534.pdf
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Effets d'un mélange de pesticides sur les hémocytes d'huître creuse, Crassostrea gigas. ArchiMer
Gagnaire, Beatrice; Geret, Florence; Haure, Joel; Martin, Jean-louis; Burgeot, Thierry; Thomas-guyon, Hélène; Renault, Tristan.
Shellfish industry is often realised in estuarine areas, subjected to pollutions due to agricultural activities. The harmful effects of pesticides on animals inhabiting these estuarine zones are poorly documented. Bivalve molluscs represent an interesting model because they are sedentary and they filter water intensively. Effects of pesticides on haemocytes functions were tested in Pacific oyster, Crassostrea gigas and analysed by flow cytometry. A combination of pesticide at environmental concentrations was tested in vivo. Animals used corresponded to two genetically selected populations produced in hatchery.
Tipo: Text Palavras-chave: Haemocytes; Flow cytometry; Pesticides; Crassostrea gigas.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-924.pdf
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Environmental stress in the pacific oyster Crassostrea gigas during gametogenesis : role of herbicides? ArchiMer
Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F..
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was...
Tipo: Text Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf
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Essai de décontamination à l'échelle professionnelle de moules rendues toxiques par le phytoplancton ArchiMer
Masson, Daniel; Ratiskol, Gilles; Burgeot, Thierry; Margat, Sylvie.
Les secteurs conchylicoles atteints par les efflorescences phytoplanctoniques toxiques sont fermés par l'Administration dès que le taux de contamination devient dangereux pour la consommation humaine. Cette fermeture peut durer plusieurs mois empêchant les conchyliculteurs de commercialiser leurs produits, ce qui a des conséquences économiques parfois graves. Il n'existe jusqu'à présent aucune possibilité technique de décontaminer ces coquillages en grande quantité et dans des délais économiquement viables. L'étude entreprise visait à mettre au point une technique permettant aux professionnels de décontaminer des coquillages rapidement (quinze jours au moins) en milieu contrôlé (bassins). L'hypothèse testée était que des coquillages placés dans un milieu...
Tipo: Text Palavras-chave: Coquillages; Décontamination; Toxicité; Phytoplancton.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00134/24499/22527.pdf
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Etude l'écotoxicité des sédiments dragues - Documents de synthèse et annexes - Projet du groupe GEODE ArchiMer
Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne.
La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude.
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf
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Evaluation du descripteur 8 « Contaminants dans le milieu » en France Métropolitaine. ArchiMer
Mauffret, Aourell; Chiffoleau, Jean-francois; Burgeot, Thierry; Wessel, Nathalie; Brun, Melanie.
Good Environmental Status (GES) for descriptor 8 of MSFD is reached when concentrations of contaminants are at levels not giving rise to pollution effects. According to the decision 2017/848/CE, descriptor 8 is defined by four criteria: concentration in the relevant matrices (sediment, biota) (D8C1), effects of chronic contamination to the marine organisms (D8C2), duration and spatial extent of acute pollution events (D8C3), effects of acute contamination to the marine organisms (D8C4). The GES for descriptor 8 is assessed at the level of four marine subregions (SRM), including: Channel – North Sea (MMN), Celtics Seas (MC), Bay of Biscay (GdG) and the Occidental Mediterranean Sea (MO). In order to achieve GES, each indicator of descriptor 8 should respect...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00461/57294/59374.pdf
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Expertise de l'Ifremer sur la contamination significative historique en milieu marin, en particulier par des métaux toxiques tels que le mercure et l'arsenic par la société Alteo ArchiMer
Dreves, Luc; Amouroux, Isabelle; Gonzalez, Jean-louis; Knoery, Joel; Andral, Bruno; Chiffoleau, Jean-francois; Brach-papa, Christophe; Claisse, Didier; Burgeot, Thierry.
Expertise réalisée dans le cadre de la saisine, datée du 10 octobre 2014, auprès du PDG de l'Ifremer de la Ministre de l’Écologie, du Développement Durable et de l’Énergie, sur la demande de poursuite de rejet d'effluents industriels liquides en Méditerranée
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37099/35615.pdf
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Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre.
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials...
Tipo: Text Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf
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Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast ArchiMer
Cachot, Jerome; Cherel, Yan; Larcher, Thibaut; Pfohl-leszkowicz, Annie; Laroche, Jean; Quiniou, Louis; Morin, Jocelyne; Schmitz, Julien; Burgeot, Thierry; Pottier, Didier.
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of...
Tipo: Text Palavras-chave: Epidemiological study; European flounder; Seine estuary; Liver histopathology; Necrosis; FCA; Tumors; Inflammations; Parasites; DNA adducts; Pollution.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23576/21498.pdf
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How can we quantify impacts of contaminants in marine ecosystems? The ICON project ArchiMer
Hylland, Ketil; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Robinson, Craig D.; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Matthew J..
An international workshop on marine integrated contaminant monitoring (ICON) was organised to test a framework on integrated environmental assessment and simultaneously assess the status of selected European marine areas. Biota and sediment were sampled in selected estuarine, inshore and offshore locations encompassing marine habitats from Iceland to the Spanish Mediterranean. The outcome of the ICON project is reported in this special issue as method-oriented papers addressing chemical analyses, PAH metabolites, oxidative stress, biotransformation, lysosomal membrane stability, genotoxicity, disease in fish, and sediment assessment, as well as papers assessing specific areas. This paper provides a background and introduction to the ICON project, by...
Tipo: Text Palavras-chave: ICON; Contaminants; North sea; Mediterranean sea; Biological effects; Biomarkers; Monitoring.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00297/40782/44970.pdf
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Hydrocarbon pollution along Moroccan coasts and BPH activity in the mussel Perna perna ArchiMer
Azdi, M.; Moukrim, A.; Burgeot, Thierry; Budzinski, H.; Chiffoleau, Jean-francois; Kaaya, A.; Zekhnini, A.; Narbonne, J. F.; Guarrigues, Ph..
Specimens of the mussel Perna perna were collected along Moroccan coasts to determine the concentrations of polycyclic aromatic hydrocarbons ( PAHs) bioaccumulated in the tissues, and to measure benzo[ a] pyrene hydroxylase (BPH) activity. Chemical analysis of PAHs show that the Mediterranean (Nador, Martil, Tanger) and central Atlantic coasts ( from Rabat to Jorf Lihoudi) are those most contaminated ( 351 245 ng.g(-1) dry weight in Tanger). The mussel contaminants were of mixed origin for most of the locations with non negligible inputs of petrogenic origin in many of them. Baseline levels of PAHs were between 6 and 55 ng.g(-1) dry weight. BPH activity showed significant correlation (r(s) = 0.64, P < 0.05) with total PAH concentrations at the six most...
Tipo: Text Palavras-chave: Mussel; Perna perna; Moroccan coasts; Polycyclic aromatic hydrocarbons; Benzoapyrene hydroxylase; Baseline level.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00000/10995/12929.pdf
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In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families. ArchiMer
Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf
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Induction and elimination of bulky benzo[a]pyrene-related DNA adducts and 8-oxodGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated feed ArchiMer
Akcha, Farida; Burgeot, Thierry; Budzinski, H; Pfohla Leszkowicz, A; Narbonne, J.
With the aim of developing biomarkers of polycyclic aromatic hydrocarbon (PAH) genotoxicity for biomonitoring of the marine environment, the formation of DNA adducts and oxidative DNA damage was studied in the sentinel organism Mytilus. Mussels M. galloprovincialis were exposed for 21 d to different doses of benzo[a]pyrene (B[a]P) (50 and 100 mg B[a]P kg(-1) dry wt mussel d(-1)) via trophic intake. The kinetics of B[a]P concentration in whole mussel tissues was determined in both groups, and a dose-response study was conducted for both gill B[a]P-related DNA adducts and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in gill and digestive gland DNA. Exposure was followed by a 10 d depuration period to study mussel recovery. Although B[a]P was...
Tipo: Text Palavras-chave: DNA repair; 8 oxodGuo; DNA adducts; Mussel; BaP.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-813.pdf
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Integrated chemical and biological assessment of contaminant impacts in selected european coastal and offshore marine areas ArchiMer
Hylland, Ketil; Robinson, Craig D.; Burgeot, Thierry; Martinez-gomez, Concepcion; Lang, Thomas; Svavarsson, Jorundur; Thain, John E.; Vethaak, A. Dick; Gubbins, Mattew J..
This paper reports a full assessment of results from ICON, an international workshop on marine integrated contaminant monitoring, encompassing different matrices (sediment, fish, mussels, gastropods), areas (Iceland, North Sea, Baltic, Wadden Sea, Seine estuary and the western Mediterranean) and endpoints (chemical analyses, biological effects). ICON has demonstrated the use of a framework for integrated contaminant assessment on European coastal and offshore areas. The assessment showed that chemical contamination did not always correspond with biological effects, indicating that both are required. The framework can be used to develop assessments for EU directives. If a 95% target were to be used as a regional indicator of MSFD GES, Iceland and offshore...
Tipo: Text Palavras-chave: ICON; Contaminants; European seas; Biological effects; Assessment.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00335/44583/44301.pdf
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Integrated indicator framework and methodology for monitoring and assessment of hazardous substances and their effects in the marine environment ArchiMer
Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil.
Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for...
Tipo: Text Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf
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Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp, in Seine Bay: A key step towards applying biological effects to monitoring ArchiMer
Burgeot, Thierry; Akcha, Farida; Menard, Dominique; Robinson, Craig; Loizeau, Veronique; Brach-papa, Christophe; Martinez-gomez, Concepcion; Le Goff, Jeremie; Budzinski, Helene; Le Menach, Karine; Cachot, Jerome; Minier, Christophe; Broeg, Katja; Hylland, Ketil.
The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological interpretation thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the comet assay, DNA adducts, micronucleus (MN), PAH metabolites, intersex and fish external pathologies were analyzed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons...
Tipo: Text Palavras-chave: Biomarkers; Bioassays; Chemical contamination; Integrated monitoring in chemistry and biology; Local study; Large geographical scale.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46789/46655.pdf
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Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis ArchiMer
Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf
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La surveillance de la qualité du milieu marin : l'expérience du Réseau National d'Observation (RNO) et le développement de biomarqueurs ArchiMer
Amiard-triquet, Claude; Burgeot, Thierry; Claisse, Didier.
Monitoring of marine environment quality can be based on chemical analysis of contaminants in the major components of the environment: seawater, sediment, biota. The respective interest of these different matrices is discussed and the fundamentals for the use of bivalves in the international Mussel Watch are presented. The objectives of the French RNO are 1) to assess the levels and trends of contaminants; 2) to assess the biological effects of contaminants. Analysis of the chemicals in biota is an indispensable complement to the biological approach, allowing their bioavailable fraction to be quantified. However, only a fraction of xenobiotics incorporated in living beings is biologically active and can exert a noxious effect on the biota. In addition,...
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00017/12848/9793.pdf
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L'éthoxyrésorufine-o-déethylase, les adduits à l'ADN et les mocronuclei dans les organismes marins. Application à la surveillance des effets biologiques sur les côtes françaises ArchiMer
Burgeot, Thierry.
Three biomarkers [ethoxyresorufm-o-deethylase (EROD), DNA adducts and micronuclei (MN) were studied for biomonitoring applications along French coasts. EROD was developed at an experirnental stage in two pilot sites, Seine Bay and Fos sur Mer, using the dab (Limanda limanda), dragon et (Callionymus lyra), red mullet (Mullus barbatus) and comber (Serranus hepatus) as target species. Higbest EROD activity was observed in the subestuarial part of Seine Bay and at the stations closest to sources of pollution from Cortiou and Fos sur Mer. The suitable number of fish for detection of a significant difference between two EROD activities was deterrnined as a function of sampling variance and a preselected statistical power. A preliminary study on hepatocytes in a...
Tipo: Text Palavras-chave: Ethoxyrésorufine-o-déethylase; Adduits à l'ADN; Micronuclei; Poisson; Moule; Huître; Biosurveillance; Ethoryresorufin-o-deethylase; DNA adducts; Micronuclei; Fish; Mussel; Oyster; Biomonitoring.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00443/55462/56984.pdf
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Monitoring of pollutant biochemical effects on marine organisms of the french coasts ArchiMer
Galgani, Francois; Bocquene, Gilles; Truquet, Philippe; Burgeot, Thierry; Chiffoleau, Jean-francois; Claisse, Didier.
The establishment of a network to assess pollutant effects is a current concern of international bodies such as the North Sea Task Force, the International Council for the Exploration of the Sea and the Intergovernmental Oceanographic Commission. With respect to the French coasts, three major considerations have been defined : the choice of monitoring areas ; the determination of a target species according to different criteria such as wide distribution, limited migration and contact with pollutant-rich sediments; and the selection of suitable parameters. This paper concerns the strategy adopted for the French coast. Ethoxyresorufin-O-deethylase, a specific cytochrome P450-dependent monooxygenase, measured by a simple microplate assay, was selected as an...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/00100/21169/18787.pdf
Registros recuperados: 62
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